The substantiation of the temperature regime of the freezing-thawing disintegration technology of C. albicans fungal cells

In this article, different temperature regimes of the freezing-thawing technology for disintegration C. albicans fungal cells have been studied. The biomass in 10 mL sterile isotonic 0.9% sodium chloride solution a Petri dish was subjected to destruction using five-fold freeze-thaw cycle following ranges: from (–25 ± 2) °C (25 °C, (–30 °С (30 and (–20 (20 °С. order find optimal each case, content proteins, polysaccharides, monosaccharides determined. According results obtained, it has determined that regime is most promising since provides maximum release polysaccharides proteins shortest possible time with minimal energy consumption.